Rv0901 from Mycobacterium tuberculosis, a possible novel virulent gene proved through the recombinant Mycobacterium smegmatis.

@article{Zhang2009Rv0901FM,
  title={Rv0901 from Mycobacterium tuberculosis, a possible novel virulent gene proved through the recombinant Mycobacterium smegmatis.},
  author={Le Zhang and Qi Zhong and Lang Bao and Ying Zhang and Lei Gao and Bi H Huang and Hui-dong Zhang},
  journal={Japanese journal of infectious diseases},
  year={2009},
  volume={62 1},
  pages={26-31}
}
The function of protein-coding gene Rv0901 of Mycobacterium tuberculosis, which belongs to the cell wall and cell processes category, is not yet clear. To explore its features, we amplified this gene from the H37Rv genome, and His-tagged Rv0901 protein was expressed and purified. Also, a recombinant plasmid bearing Rv0901 was constructed and electroporated into a virulent Mycobacterium smegmatis, using shuttle expression vector pMV261. Transformants were induced to express a predicted protein… CONTINUE READING
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