Ruthenium red-mediated inhibition of large-conductance Ca2+-activated K+ channels in rat pituitary GH3 cells.

@article{Wu1999RutheniumRI,
  title={Ruthenium red-mediated inhibition of large-conductance Ca2+-activated K+ channels in rat pituitary GH3 cells.},
  author={Sheng-Nan Wu and Chung Ren Jan and Hui Fang Li},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1999},
  volume={290 3},
  pages={998-1005}
}
The ionic mechanism of actions of ruthenium red was examined in rat anterior pituitary GH(3) cells. In whole-cell recording experiments, ruthenium red reversibly caused an inhibition of Ca(2+)-activated K(+) current [I(K(Ca))] in a dose-dependent manner. The IC(50) value of ruthenium red-induced inhibition of I(K(Ca)) was 15 microM. Neither carbonyl cyanide m-chlorophenyl hydrazone (CCCP; 10 microM), an uncoupler of oxidative phosphorylation in mitochondria, nor cyclosporin A (200 nM), an… CONTINUE READING