Rubisco small-subunit α-helices control pyrenoid formation in Chlamydomonas.

Abstract

The pyrenoid is a subcellular microcompartment in which algae sequester the primary carboxylase, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). The pyrenoid is associated with a CO(2)-concentrating mechanism (CCM), which improves the operating efficiency of carbon assimilation and overcomes diffusive limitations in aquatic photosynthesis. Using the model alga Chlamydomonas reinhardtii, we show that pyrenoid formation, Rubisco aggregation, and CCM activity relate to discrete regions of the Rubisco small subunit (SSU). Specifically, pyrenoid occurrence was shown to be conditioned by the amino acid composition of two surface-exposed α-helices of the SSU: higher plant-like helices knock out the pyrenoid, whereas native algal helices establish a pyrenoid. We have also established that pyrenoid integrity was essential for the operation of an active CCM. With the algal CCM being functionally analogous to the terrestrial C(4) pathway in higher plants, such insights may offer a route toward transforming algal and higher plant productivity for the future.

DOI: 10.1073/pnas.1210993109
05010020132014201520162017
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@article{Meyer2012RubiscoS, title={Rubisco small-subunit α-helices control pyrenoid formation in Chlamydomonas.}, author={Moritz T. Meyer and Todor Genkov and Jeremy N. Skepper and Juliette Jouhet and Madeline C. Mitchell and Robert J. Spreitzer and Howard Griffiths}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2012}, volume={109 47}, pages={19474-9} }