Routine preparation of air-dried negatively stained and unstained specimens on holey carbon support films: a review of applications.

@article{Harris2002RoutinePO,
  title={Routine preparation of air-dried negatively stained and unstained specimens on holey carbon support films: a review of applications.},
  author={J. Robin Harris and Dirk Scheffler},
  journal={Micron},
  year={2002},
  volume={33 5},
  pages={461-80}
}
Several representative examples are given of the successful application of negative staining across the holes of holey carbon support films using 5% (w/v) ammonium molybdate solution containing trehalose. The inclusion of 0.1% (w/v) trehalose is considered to be most satisfactory, although good data have also been obtained in the presence of 0.01 and 1.0… CONTINUE READING