Routine monitoring of Cryptosporidium oocysts in water using flow cytometry.

  title={Routine monitoring of Cryptosporidium oocysts in water using flow cytometry.},
  author={G Vesey and J. S. Slade and Maria Byrne and Kristen P Shepherd and Patrick J. Dennis and Crystal C. R. Fricker},
  journal={The Journal of applied bacteriology},
  volume={75 1},
A flow cytometric method for the routine analysis of environmental water samples for the presence of Cryptosporidium oocysts has been developed. It uses a Coulter Epics Elite flow cytometer to examine water samples and to separate oocysts from contaminating debris by cell sorting. The sorted particles are then rapidly screened by microscopy. The method has been evaluated and compared with direct epifluorescence microscopy on 325 river, reservoir and drinking water samples. The technique was… CONTINUE READING
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