Routine heat inactivation of serum reduces its capacity to promote cell attachment

  title={Routine heat inactivation of serum reduces its capacity to promote cell attachment},
  author={Donald J. Giard},
  journal={In Vitro Cellular & Developmental Biology},
Heat inactivation (56°C for 40 min) of bovine calf serum was shown to diminish its capacity to promote the attachment of cells to plastic or glass surfaces. This effect was not observed in stationary cultures (culture dishes) but became manifest under conditions in which the cells were subjected to a small amount of liquid shear force, i.e. by growing cells in roller bottles or culture tubes. Of four cell lines tested on bovine calf serum (SV-BHK, BALB-3T3, CV-1, and FS-4) SV-BHK and CV-1 cells… CONTINUE READING
5 Citations
26 References
Similar Papers


Publications referenced by this paper.
Showing 1-10 of 26 references

Thermal stability of human plasma fibroneetin and its constituent domains

  • K. C. Ingham, S. A. Brew, Broekselmann, J T., etal
  • J. Biol. Chem
  • 1984

Proteins from fibroblast conditioned medium mediate and enhance cell adhesion, spreading and growth

  • M. Yamada, N. Ikegami, T. Okegami
  • Proc. Jpn. Acad. 58B:160-164;
  • 1982

Structure and function of fibronectin

  • K. M. Yamada, M. Hayashi, S. K. Akiyama
  • Extra cellular matrix
  • 1982
1 Excerpt

Similar Papers

Loading similar papers…