Routine Cryopreservation of Isolates of Steinernema and Heterorhabditis spp.

@article{Curran1992RoutineCO,
  title={Routine Cryopreservation of Isolates of Steinernema and Heterorhabditis spp.},
  author={James Andrew Curran and Christine Gilbert and Kris Butler},
  journal={Journal of nematology},
  year={1992},
  volume={24 2},
  pages={
          269-70
        }
}
Infective-stage juveniles of Steinernema and Heterorhabditis spp. were cryopreserved using two-stage incubation in glycerol and 70% methanol before storage in cryotubes in liquid nitrogen. Optimal glycerol concentrations and incubation times for survival were determined for different species, but acceptable survival of all species and isolates of entomopathogenic nematodes can be obtained using 15% (w/w) glycerol and incubation for 48 hours. Mean survival was 69% for isolates of Steinernema and… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 19 CITATIONS

Cryopreservation of Embryos from Model Animals and Human

VIEW 4 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-2 OF 2 REFERENCES

Commercial storage and shipment of entomogenous nematodes

I. Popiel, K. D. Holtemann, I. Glazer, Womersley
  • World International Property Organization, International Patent No
  • 1988
VIEW 1 EXCERPT

Similar Papers