Rough mutants defective in core and O-polysaccharide synthesis and export induce antibodies reacting in an indirect ELISA with smooth lipopolysaccharide and are less effective than Rev 1 vaccine against Brucella melitensis infection of sheep.

@article{Barrio2009RoughMD,
  title={Rough mutants defective in core and O-polysaccharide synthesis and export induce antibodies reacting in an indirect ELISA with smooth lipopolysaccharide and are less effective than Rev 1 vaccine against Brucella melitensis infection of sheep.},
  author={Maria Bel{\'e}n Barrio and Mar{\'i}a Jes{\'u}s Grill{\'o} and Pilar Munoz and Isabelle Jacques and David Gonz{\'a}lez and Mar{\'i}a-Jes{\'u}s de Miguel and Clara Mar{\'i}a Mar{\'i}n and Montserrat Barber{\'a}n and Jean-J Letesson and Jean-P Gorvel and Ignacio Moriy{\'o}n and Jos{\'e} Mar{\'i}a Lozano Blasco and Michel S. Zygmunt},
  journal={Vaccine},
  year={2009},
  volume={27 11},
  pages={1741-9}
}
Classical brucellosis vaccines induce antibodies to the O-polysaccharide section of the lipopolysaccharide that interfere in serodiagnosis. Brucella rough (R) mutants lack the O-polysaccharide but their usefulness as vaccines is controversial. Here, Brucella melitensis R mutants in all main lipopolysaccharide biosynthetic pathways were evaluated in sheep in… CONTINUE READING