Rotavirus and Adenovirus are the majority of the viral factors of acute gastroenteritis which is responsible for child mortality. These viruses are the most common reasons for the viral gastroenteritis in children aged 6-24 months. The aim of this study is to determine the age, seasonal distribution and the positivity rate of Rotavirus and Adenovirus infections among children with diarrhea who were admitted to a tertiary care education and research hospital. The 5412 children (2992 (55%) male and 2420 female (45%)) between the ages of 0-6 were included in the study. The data of them were obtained retrospectively between January 2013 and January 2015 from the results of laboratory reports. Rotavirus Ag in 395 (7.2%) and Adenovirus Ag in 20 (0.36%) of the patients were studied and 7 (0.12%) of them were determined as positive for both factors. It was determined that Rotavirus positivity was higher in patients during the colder months compared to others. Positive cases were observed mostly in children between the ages of 0 and 2, followed by the group of children who were 1-year old. As a result, clinician should remember rotavirus infections in pediatric gastroenteritis which are observed particularly in winter, according to the distinctive diagnosis. It is decided that Rotavirus Ag-ELISA tests are applicable and sensitive in the diagnosis of the Rotavirus infection. Thus, unnecessary antibiotic usage can be prevented with the help of a sensitive diagnosis of the infection.