Rotational dynamics of type I Fc epsilon receptors on individually-selected rat mast cells studied by polarized fluorescence depletion.

@article{Rahman1992RotationalDO,
  title={Rotational dynamics of type I Fc epsilon receptors on individually-selected rat mast cells studied by polarized fluorescence depletion.},
  author={Normastura Abdul Rahman and Israel Pecht and Deborah A. Roess and B. George Barisas},
  journal={Biophysical journal},
  year={1992},
  volume={61 2},
  pages={334-46}
}
We report the first application of polarized fluorescence depletion (PFD), a technique which combines the sensitivity of fluorescence detection with the long lifetimes of triplet probes, to the measurement of membrane protein rotational diffusion on individually selected, intact mammalian cells. We have examined the rotation of type I Fc epsilon receptors (Fc epsilon RI) on rat mucosal mast cells of the RBL-2H3 line in their resting monomeric and differently oligomerized states using as probes… CONTINUE READING