Room-temperature detection of a single molecule's absorption by photothermal contrast.

@article{Gaiduk2010RoomtemperatureDO,
  title={Room-temperature detection of a single molecule's absorption by photothermal contrast.},
  author={Alexander Gaiduk and Mustafa Yorulmaz and Paul V Ruijgrok and Michel Orrit},
  journal={Science},
  year={2010},
  volume={330 6002},
  pages={353-6}
}
So far, single-molecule imaging has predominantly relied on fluorescence detection. We imaged single nonfluorescent azo dye molecules in room-temperature glycerol by the refractive effect of the heat that they release in their environment upon intense illumination. This photothermal technique provides contrast for the absorbing objects only, irrespective of scattering by defects or roughness, with a signal-to-noise ratio of ~10 for a single molecule in an integration time of 300 milliseconds… CONTINUE READING

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