Roles of transcription and repair in alkylation mutagenesis.

  title={Roles of transcription and repair in alkylation mutagenesis.},
  author={Toshihiro Ito and Tsuneaki Nakamura and Hisaji Maki and Mutsuo Sekiguchi},
  journal={Mutation research},
  volume={314 3},
Mutations occurring in Escherichia coli cells exposed to alkylating agents have been analyzed using an assay for forward mutations in the E. coli rpsL gene cloned on a high copy number plasmid. N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutations were recovered from wild-type and O6-methylguanine methyltransferase-deficient mutant (ada- ogt-) cells and their sequence alterations determined. We found that the mutations recovered from the wild-type strain were predominantly G:C to A:T… CONTINUE READING


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