Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5.

@article{Galkin2007RolesOT,
  title={Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5.},
  author={Oleksandr Galkin and Amber A. Bentley and Sujatha Gupta and Beth-Ann Compton and Barsanjit Mazumder and Terri Goss Kinzy and William C. Merrick and Maria Hatzoglou and Tatyana V. Pestova and Christopher U. T. Hellen and Anton A. Komar},
  journal={RNA},
  year={2007},
  volume={13 12},
  pages={2116-28}
}
Ribosomal protein (rp) S5 belongs to a family of ribosomal proteins that includes bacterial rpS7. rpS5 forms part of the exit (E) site on the 40S ribosomal subunit and is essential for yeast viability. Human rpS5 is 67% identical and 79% similar to Saccharomyces cerevisiae rpS5 but lacks a negatively charged (pI approximately 3.27) 21 amino acid long N-terminal extension that is present in fungi. Here we report that replacement of yeast rpS5 with its human homolog yielded a viable yeast strain… CONTINUE READING