Corpus ID: 7769987

Roles of conserved ectodomain cysteines of the rat P2X4 purinoreceptor in agonist binding and channel gating.

@article{Rokic2010RolesOC,
  title={Roles of conserved ectodomain cysteines of the rat P2X4 purinoreceptor in agonist binding and channel gating.},
  author={M. Rokic and V. Tvrdoňov{\'a} and V. V{\'a}vra and M. Jindřichov{\'a} and T. Obsil and S. Stojilkovic and H. Zemkova},
  journal={Physiological research},
  year={2010},
  volume={59 6},
  pages={
          927-35
        }
}
Mammalian P2X receptors contain 10 conserved cysteine residues in their ectodomains, which form five disulfide bonds (SS1-5). Here, we analyzed the relevance of these disulfide pairs in rat P2X4 receptor function by replacing one or both cysteines with alanine or threonine, expressing receptors in HEK293 cells and studying their responsiveness to ATP in the absence and presence of ivermectin, an allostenic modulator of these channels. Response to ATP was not altered when both cysteines forming… Expand
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