Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli.

@article{Han2004RolesAA,
  title={Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli.},
  author={M. Han and Si Jae Park and Tae Jung Park and Sang Yup Lee},
  journal={Biotechnology and bioengineering},
  year={2004},
  volume={88 4},
  pages={426-36}
}
Proteome profiling of the inclusion body (IB) fraction of recombinant proteins produced in Escherichia coli suggested that two small heat shock proteins, IbpA and IbpB, are the major proteins associated with IBs. In this study, we demonstrate that IbpA and IbpB facilitate the production of recombinant proteins in E. coli and play important roles in protecting recombinant proteins from degradation by cytoplasmic proteases. We examined the cytosolic production, and Tat- or Sec-dependent secretion… CONTINUE READING