Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation.

@article{Rodighiero2002RoleOU,
  title={Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation.},
  author={Chiara Rodighiero and Billy Tsai and Tom A Rapoport and Wayne I Lencer},
  journal={EMBO reports},
  year={2002},
  volume={3 12},
  pages={1222-7}
}
Cholera toxin travels from the cell surface of affected mammalian cells to the endoplasmic reticulum (ER), where the A1 chain is released and retro-translocated across the ER membrane into the cytosol. We have tested whether, as in other cases, retro-translocation requires poly-ubiquitination. We show that an A1 chain mutant that lacks lysines and has a blocked N-terminus, and therefore cannot be ubiquitinated, remains active in vivo. The A1 chain is not degraded in the cytosol, as demonstrated… CONTINUE READING

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