Role of the major ecto-phosphoprotein in sperm flagellar motility using a cell electroporation method.

@article{Maiti2008RoleOT,
  title={Role of the major ecto-phosphoprotein in sperm flagellar motility using a cell electroporation method.},
  author={Arunima Maiti and K. P. Mishra and Gopal Chandra Majumder},
  journal={Molecular reproduction and development},
  year={2008},
  volume={75 7},
  pages={
          1185-95
        }
}
Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the help of cell electroporation technique to investigate the effect of MPS on sperm flagellar motility. The optimum conditions for electroporation of sperm cells consisted of exposure… CONTINUE READING

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