Role of the individual domains of translation termination factor eRF1 in GTP binding to eRF3.

@article{Kononenko2008RoleOT,
  title={Role of the individual domains of translation termination factor eRF1 in GTP binding to eRF3.},
  author={Artem V. Kononenko and Vladimir A Mitkevich and Vera I. Dubovaya and Peter Kolosov and Alexander A. Makarov and L. L. Kisselev},
  journal={Proteins},
  year={2008},
  volume={70 2},
  pages={388-93}
}
Eukaryotic translational termination is triggered by polypeptide release factors eRF1, eRF3, and one of the three stop codons at the ribosomal A-site. Isothermal titration calorimetry shows that (i) the separated MC, M, and C domains of human eRF1 bind to eRF3; (ii) GTP binding to eRF3 requires complex formation with either the MC or M + C domains; (iii) the M domain interacts with the N and C domains; (iv) the MC domain and Mg2+ induce GTPase activity of eRF3 in the ribosome. We suggest that… CONTINUE READING

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