• Chemistry, Medicine
  • Published in
    The Journal of biological…
    1984

Role of the gamma subunit of chloroplast coupling factor 1 in the light-dependent activation of photophosphorylation and ATPase activity by dithiothreitol.

@article{Ketcham1984RoleOT,
  title={Role of the gamma subunit of chloroplast coupling factor 1 in the light-dependent activation of photophosphorylation and ATPase activity by dithiothreitol.},
  author={Stuart R. Ketcham and James W. Davenport and Kurt Warncke and Richard E. Mccarty},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 11},
  pages={
          7286-93
        }
}
In leaves and intact chloroplasts, oxidation and reduction have been shown previously to regulate the ATPase activity of thylakoids. Illumination of spinach chloroplast thylakoids in the presence of dithiothreitol, which activates the ability of thylakoids to catalyze sustained ATP hydrolysis in the dark, causes increased incorporation of N-ethylmaleimide into the gamma subunit of coupling factor 1 (CF1). A disulfide bond in the gamma subunit is reduced during activation. The residues involved… CONTINUE READING

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