Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.

@article{Himeno1989RoleOT,
  title={Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.},
  author={Hyouta Himeno and Tsunemi Hasegawa and Takuya Ueda and Kimitsuna Watanabe and K. T. Miura and Masato Shimizu},
  journal={Nucleic acids research},
  year={1989},
  volume={17 19},
  pages={7855-63}
}
All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate the role of this unique structure in aminoacylation, we constructed in vitro transcripts corresponding to the E. coli histidine tRNA sequence and its variants at the G-1-C73 base pair, by using T7 RNA polymerase transcription system. A transcript having a wild-type sequence with no modified bases was a good substrate for histidyl-tRNA synthetase (HisRS), and aminoacylation… CONTINUE READING
26 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 26 extracted citations

Similar Papers

Loading similar papers…