Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts

@article{Anderson1977RoleOT,
  title={Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts},
  author={Richard G. W. Anderson and Michael S Brown and J. Goldstein},
  journal={Cell},
  year={1977},
  volume={10},
  pages={351-364}
}
125I-labeled and ferritin-labeled low density lipoprotein (LDL) were used as visual probes to study the surface distribution of LDL receptors and to examine the mechanism of the endocytosis of this lipoprotein in cultured human fibrobasts. Light microscopic autoradiograms of whole cells incubated with 125I-LDL at 4 degrees C showed that LDL receptors were widely but unevenly distributed over the cell surface. With the electron microscope, we determined that 60-70% of the ferritin-labeled LDL… Expand
Binding and internalization of 125I-LDL in normal and mutant human fibroblasts. A quantitative autoradiographic study.
TLDR
Results obtained with ferritin-labeled LDL are confirmed and illustrate the complementary application of two different morphologic probes, each of which offers special advantages for special problems. Expand
Fluorescence visualization of receptor-bound low density lipoprotein in human fibroblasts.
AbstractPrevious electron microscopic studies have shown that receptors for plasma low density lipoprotein (LDL) in human fibroblasts are located predominantly in coated pits on the cell surface. InExpand
Display of low density lipoprotein receptors is clustered, not dispersed, in fibroblast and hepatocyte plasma membranes.
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Histochemical Characterization of Low Density Lipoprotein Receptors in Internalization‐Defective Familial Hypercholesterolemia
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The results indicate that the internalization defect in MN's cells is the same as that in the GM2408A cells; neither can localize the LDL and LDL receptor complex in coated pits. Expand
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Work in several laboratories has indicated that exposure to a low-pH environment within the cell could account for many aspects of the sorting of ligands and receptors. Expand
Co-localization of 125I-epidermal growth factor and ferritin-low density lipoprotein in coated pits: a quantitative electron microscopic study in normal and mutant human fibroblasts
TLDR
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TLDR
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TLDR
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References

SHOWING 1-10 OF 34 REFERENCES
Localization of low density lipoprotein receptors on plasma membrane of normal human fibroblasts and their absence in cells from a familial hypercholesterolemia homozygote.
TLDR
The present ultrastructural data are consistent with previous biochemical and genetic evidence indicating that LDL exerts its regulatory action on cellular cholesterol metabolism in fibroblasts through an interaction with a specific cell surface receptor and that this receptor is defective in homozygous familial hypercholesterolemia fibro Blasts. Expand
Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans
TLDR
The physiologic importance of the cell surface LDL receptor was emphasized by the finding that mutant fibroblasts from a subject with homozygous Familial Hypercholesterolemia did not show cell surface binding of 125I-LDL, as measured by heparin release, at either 4 degrees C or 37 degrees C. Expand
Role of bristle-coated membrane in the uptake of ferritin by rat macrophages.
TLDR
Counts of bristle-coated membrane sites before and after exposure to ferritin suggest that ferrit in rat peritoneal macrophages is selectively bound to preformed patches ofBristle- coated surface membrane which subsequently invaginate to form the coated vesicles. Expand
Regulation of the activity of the low density lipoprotein receptor in human fibroblasts
TLDR
C cultured human fibroblasts regulate their intracellular cholesterol content by regulating the activity of the LDL receptor, which in turn controls the rate of cellular entry of cholesterol derived from plasma LDL contained within the culture medium. Expand
THE LOW‐DENSITY LIPOPROTEIN PATHWAY IN HUMAN FIBROBLASTS: RELATION BETWEEN CELL SURFACE RECEPTOR BINDING AND ENDOCYTOSIS OF LOW‐DENSITY LIPOPROTEIN *
TLDR
The physiologic importance of the LDL pathway is indicated by the fact that patients who lack the LDL receptor (FH homozygotes) develop both profound hyper-cholesterolemia and fulminant atherosclerosis. Expand
FUNCTIONS OF COATED VESICLES DURING PROTEIN ABSORPTION IN THE RAT VAS DEFERENS
TLDR
Large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and some small coated vedicle serve as primary lysOSome to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies. Expand
Binding, internalization, and degradation of low density lipoprotein by normal human fibroblasts and by fibroblasts from a case of homozygous familial hypercholesterolemia.
Skin fibroblasts from a patient with homozygous familial hypercholesterolemia (HFH) were compared with normal skin fibroblasts with regard to binding, internalization, and degradation of iodinatedExpand
Analysis of a mutant strain of human fibroblasts with a defect in the internalization of receptor-bound low density lipoprotein
TLDR
The current data indicate that at least two gene products are necessary for the adsorptive endocytosis of LDL: one that is required for the binding of the lipoprotein, and one that promotes the internalization of the receptor-bound ligand. Expand
PINOCYTOSIS IN FIBROBLASTS
TLDR
In spite of the availability of an accurate quantitative assay for fluid and solute uptake, the function of pinocytosis in tissue culture cells remains unknown. Expand
Binding and degradation of low density lipoproteins by cultured human fibroblasts. Comparison of cells from a normal subject and from a patient with homozygous familial hypercholesterolemia.
TLDR
It is raised that a prerequisite for the regulation of cholestero-genesis in cultured fibroblasts is the initial binding of low density lipoproteins to the high affinity surface receptor sites and that a defect in this process represents the primary genetic abnormality in the disorder familial hypercholesterolemia. Expand
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