Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts

@article{Anderson1977RoleOT,
  title={Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts},
  author={Richard G. W. Anderson and Michael S. Brown and Joseph L. Goldstein},
  journal={Cell},
  year={1977},
  volume={10},
  pages={351-364}
}

Fluorescence visualization of receptor-bound low density lipoprotein in human fibroblasts.

AbstractPrevious electron microscopic studies have shown that receptors for plasma low density lipoprotein (LDL) in human fibroblasts are located predominantly in coated pits on the cell surface. In

Display of low density lipoprotein receptors is clustered, not dispersed, in fibroblast and hepatocyte plasma membranes.

A comparison of LDL and beta-very low density lipoprotein receptor distribution in fibroblasts and hepatocytes suggests that this cluster pattern is a characteristic of the LDL (apolipop Protein B/E) receptor across cell types, but that the closely related apolipoprotein E receptor differs in that it is inserted individually in a highly dispersed state.

Histochemical Characterization of Low Density Lipoprotein Receptors in Internalization‐Defective Familial Hypercholesterolemia

The results indicate that the internalization defect in MN's cells is the same as that in the GM2408A cells; neither can localize the LDL and LDL receptor complex in coated pits.

ACIDIFICATION OF ENDOCYTIC VESICLES AND THE INTRACELLULAR PATHWAYS OF LIGANDS AND RECEPTORS a

Work in several laboratories has indicated that exposure to a low-pH environment within the cell could account for many aspects of the sorting of ligands and receptors.

Co-localization of 125I-epidermal growth factor and ferritin-low density lipoprotein in coated pits: a quantitative electron microscopic study in normal and mutant human fibroblasts

Quantitative studies in normal cells showed that 75% of the coated pits and vesicles that contained 125I-EGF also contained ferritin-LDL, indicating that both ligands enter the cell through the same endocytotic vesicle.

Conjugates of colloidal gold with native and acetylated low density lipoproteins for ultrastructural investigations on receptor-mediated endocytosis by cultured human monocyte-derived macrophages

The morphological aspects of the endocytosis of LDL and AcLDL by cultured human monocyte-derived macrophages are similar, and cells incubated at 37° C for various time periods showed internalization of both LDL- andAcLDL-gold complexes via small coated and non-coated vesicles and processing of the complexes in smooth-walled endosomes.

Surface distribution and recycling of the low density lipoprotein receptor as visualized with antireceptor antibodies

The mechanism of internalization of LDL in human fibroblasts is described, using LDL that was covalently coupled to ferritin as a specific electron microscopic probe, and it was shown that LDL binds to receptors that are associated with coated pits on the cell surface.
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