Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry.

@article{Westrick1990RoleOT,
  title={Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry.},
  author={J A Westrick and Kevin S. Peters and J. Dezz Ropp and Stephen G Sligar},
  journal={Biochemistry},
  year={1990},
  volume={29 28},
  pages={6741-6}
}
The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns. The enthalpy of the intermediate increases by approximately 7 kcal/mol upon replacing arginine-45 with either asparagine or glycine. These observations support recent proposals… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…