Role of the T cell receptor alpha chain in the development and phenotype of naturally arising CD4+CD25+ T cells.

Abstract

The T cell receptor alpha chain repertoire and the possible influence of the alpha chain on the development and phenotype of naturally arising mouse CD4+CD25+ T cells have not been extensively analysed. We used all available Valpha-specific monoclonal antibodies and a sensitive multiplex genomic DNA PCR assay to study the Valpha repertoire of CD4+CD25+ T cells in normal mice. To address whether CD4+CD25+ T cells express two TCR alpha chains, we have carried out four-colour flow cytometry using combinations of the available anti-Valpha reagents in mice where one allele of the TCRA locus had been inactivated. Results indicate that the Valpha repertoire of CD4+CD25+ T cells is as diverse as their CD25- partners. In addition, CD4+CD25+ T cells develop normally in Tcralpha+/- mice and we show for the first time that despite expressing only one TCRalpha chain, they retain their characteristic CD4(low), CD3(low), TCRbeta(low), CD5(high), CD45RB(low) and cytoplasmic CD152(high) phenotype.

Cite this paper

@article{Bosco2006RoleOT, title={Role of the T cell receptor alpha chain in the development and phenotype of naturally arising CD4+CD25+ T cells.}, author={Nabil Bosco and Hsiu-Cheng Hung and Nicolas Pasqual and Evelyne Jouvin-Marche and Patrice N Marche and Nicholas R J Gascoigne and Rhodri Ceredig}, journal={Molecular immunology}, year={2006}, volume={43 3}, pages={246-54} }