Role of the N-terminus of subunit III in proton uptake in cytochrome c oxidase of Rhodobacter sphaeroides.

@article{Alnajjar2014RoleOT,
  title={Role of the N-terminus of subunit III in proton uptake in cytochrome c oxidase of Rhodobacter sphaeroides.},
  author={Khadijeh S Alnajjar and Jonathan P. Hosler and Lawrence J. Prochaska},
  journal={Biochemistry},
  year={2014},
  volume={53 3},
  pages={496-504}
}
The catalytic core of cytochrome c oxidase consists of three subunits that are conserved across species. The N-terminus of subunit III contains three histidine residues (3, 7, and 10) that are surface-exposed, have physiologically relevant pKa values, and are in close proximity of the mouth of the D-channel in subunit I. A triple-histidine mutation (to glutamine) was created in Rhodobacter sphaeroides. The mutant enzyme retains 60% of wild-type activity. Absorbance during steady-state turnover… CONTINUE READING
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