Role of the 3′ splice site consensus sequence in mammalian pre-mRNA splicing

@article{Ruskin1985RoleOT,
  title={Role of the 3′ splice site consensus sequence in mammalian pre-mRNA splicing},
  author={Barbara Ruskin and Michael R. Green},
  journal={Nature},
  year={1985},
  volume={317},
  pages={732-734}
}
Pre-mRNA splicing has been shown to occur by a two-step pathway1,2. In the first stage, the pre-mRNA is cleaved at the 5′ splice site, generating the first exon RNA species and an RNA species composed of the intron and second exon (IVS1–exon 2 RNA species). In the second stage, cleavage at the 3′ splice site and ligation of the exons occurs, resulting in the excision of the intact intron. The excised intron and IVS1–exon 2 RNA species are in the form of a lariat in which the 5′ end of the… CONTINUE READING