Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109

@article{Wang2014RoleOP,
  title={Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109},
  author={Guang-Yuan Wang and Yan Zhang and Zhe Chi and Guanglei Liu and Z. Wang and Zhenming Chi},
  journal={Applied Microbiology and Biotechnology},
  year={2014},
  volume={99},
  pages={1637-1645}
}
Yarrowia lipolytica ACA-DC 50109 is an oleaginous yeast. In order to know the function of pyruvate carboxylase (PYC) in lipid biosynthesis, the PYC gene cloned from Pichia guilliermondii Pcla22 was overexpressed in the oleaginous yeast. The lipid contents in the wild-type strain ACA-DC 50109 and the transformants P4, P7, and P103 were 30.2 % (w/w) 36.5 % (w/w), 38.2 % (w/w), and 37.9 % (w/w). However, the amount of the secreted citric acids by strains ACA-DC 50109, P4, P77, and P103 were 0.5… CONTINUE READING
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