Role of protein kinase-A in homologous down-regulation of parathyroid hormone (PTH)/PTH-related peptide receptor messenger ribonucleic acid in human osteoblast-like SaOS-2 cells.

@article{Fukayama1994RoleOP,
  title={Role of protein kinase-A in homologous down-regulation of parathyroid hormone (PTH)/PTH-related peptide receptor messenger ribonucleic acid in human osteoblast-like SaOS-2 cells.},
  author={Shoichi Fukayama and Ernestina Schipani and Harald W Jueppner and Beate Lanske and Henry M. Kronenberg and Abdul Badi Abou-Samra and F. Richard Bringhurst},
  journal={Endocrinology},
  year={1994},
  volume={134 4},
  pages={1851-8}
}
Homologous down-regulation of PTH/PTH-related peptide (PTHrP) receptor expression occurs in several PTH-responsive osteoblastic cell lines, but the mechanisms responsible are not well understood. We have used wild-type SaOS-2 human osteoblastic cells, in which homologous PTH/PTHrP receptor down-regulation occurs within 4 h, and a mutant cAMP-resistant subclone (Ca4A strain), to investigate the mechanisms by which PTH/PTHrP receptor mRNA is regulated. SaOS-2 cells expressed a single 2.2- to 2.5… CONTINUE READING