Role of oxygen radicals generated by NADPH oxidase in apoptosis induced in human leukemia cells.

@article{Hiraoka1998RoleOO,
  title={Role of oxygen radicals generated by NADPH oxidase in apoptosis induced in human leukemia cells.},
  author={Wakako Hiraoka and N Vazquez and Wilberto Nieves-Neira and Stephen J. Chanock and Yves Pommier},
  journal={The Journal of clinical investigation},
  year={1998},
  volume={102 11},
  pages={1961-8}
}
We have used a human leukemia cell line that, after homologous recombination knockout of the gp91-phox subunit of the phagocyte respiratory-burst oxidase cytochrome b-558, mimics chronic granulomatous disease (X-CGD) to study the role of oxygen radicals in apoptosis. Camptothecin (CPT), a topoisomerase I inhibitor, induced significantly more apoptosis in PLB-985 cells than in X-CGD cells. Sensitivity to CPT was enhanced after neutrophilic differentiation, but was lost after monocytic… CONTINUE READING