Role of nucleotide hydrolysis in microtubule assembly

  title={Role of nucleotide hydrolysis in microtubule assembly},
  author={R C Weisenberg and William J Deery},
MICROTUBULE assembly in vitro requires (in normal conditions) that GTP be bound to the exchangeable nucleotide-binding site of tubulin1–3. The bound GTP is hydrolysed during polymerisation and the resulting GDP remains bound to the microtubule while the phosphate is released into the medium. Although hydrolysis normally occurs during polymerisation, microtubules will form in a non-hydrolysable analogue of GTP, guanylyl imidodiphosphate (GMP-PNP)4–6. We have observed6, as also has Arai5, that… CONTINUE READING