Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production

@article{Chun1977RoleON,
  title={Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production},
  author={L L Chun and Paul H. Patterson},
  journal={The Journal of Cell Biology},
  year={1977},
  volume={75},
  pages={694 - 704}
}
To study the effect of nerve growth factor (NGF) on neuronal survival, growth, and differentiation, cultures of dissociated neonatal rat sympathetic neurons virtually free of other cell types were maintained for 3-4 wk. In the absence of NGF, the neurons did not survive for more than a day. Increased levels of NGF increased neuronal survival and growth (total protein and total lipid phosphate); saturation occurred at 0.5 microgram/ml 7S NGF. Neuronal differentiation examined by measuring… CONTINUE READING

Citations

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SHOWING 1-10 OF 40 CITATIONS

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  • The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1987
VIEW 2 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

Autonomic neural control of intrathoracic airways.

  • Comprehensive Physiology
  • 2012
VIEW 1 EXCERPT
CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 13 REFERENCES

Biological properties of a nerve-growth promoting protein and its antiserum

R. LEVI-MONTALCINI
  • ANGELETTI
  • 1961
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

The role of NGF in the development of rat sym702 THE JOURNAL OF CELL BIOLOGY

L.L.Y. CHUN, P. H. PATTERSON
  • VOLUME 75,
  • 1976
VIEW 1 EXCERPT

Surface movements during the growth of single explanted neurons.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1970

The nerve growth factor: purification as a 30,000-molecular-weight protein.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1969
VIEW 3 EXCERPTS