Corpus ID: 31817958

Role of methotrexate polyglutamylation and cellular energy metabolism in inhibition of methotrexate binding to dihydrofolate reductase by 5-formyltetrahydrofolate in Ehrlich ascites tumor cells in vitro.

@article{Matherly1983RoleOM,
  title={Role of methotrexate polyglutamylation and cellular energy metabolism in inhibition of methotrexate binding to dihydrofolate reductase by 5-formyltetrahydrofolate in Ehrlich ascites tumor cells in vitro.},
  author={L. Matherly and D. Fry and I. Goldman},
  journal={Cancer research},
  year={1983},
  volume={43 6},
  pages={
          2694-9
        }
}
  • L. Matherly, D. Fry, I. Goldman
  • Published 1983
  • Biology, Medicine
  • Cancer research
  • 5-Formyltetrahydrofolate was found to reverse the binding of methotrexate to dihydrofolate reductase in the Ehrlich ascites tumor in vitro. When cells pretreated with methotrexate were resuspended in methotrexate-free buffer containing 5-formyltetrahydrofolate (or 5-methyltetrahydrofolate), net dissociation of the antifolate from the enzyme was observed. Methotrexate associated with the enzyme under these conditions was below the enzyme binding capacity. However, glucose or azide increased the… CONTINUE READING
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