Role of membrane-bound 5'-nucleotidase in nucleotide uptake by the moderate halophile Vibrio costicola.

@article{BengisGarber1982RoleOM,
  title={Role of membrane-bound 5'-nucleotidase in nucleotide uptake by the moderate halophile Vibrio costicola.},
  author={Carmela Bengis-Garber and Donn J. Kushner},
  journal={Journal of bacteriology},
  year={1982},
  volume={149 3},
  pages={808-15}
}
Intact cells of Vibrio costicola hydrolyzed ATP, ADP, and AMP. The membrane-bound 5'-nucleotidase (C. Bengis-Garber and D. J. Kushner, J. Bacteriol. 146:24-32, 1981) was solely responsible for these activities, as shown by experiments with anti-5'-nucleotidase serum and with the ATP analog, adenosine 5'-(beta gamma-imido)-diphosphate. Fresh cell suspensions rapidly accumulated 8-14C-labeled adenine 5'-nucleotides and adenosine. The uptake of ATP, ADP, and AMP (but not the adenosine uptake) was… CONTINUE READING