Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina.

@article{Hao2014RoleOM,
  title={Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina.},
  author={Guangfei Hao and Haiqin Chen and Lei Wang and Zhennan Gu and Yuanda Song and Hao Zhang and Wei Chen and Yong Q. Chen},
  journal={Applied and environmental microbiology},
  year={2014},
  volume={80 9},
  pages={2672-8}
}
The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in Mucor circinelloides. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in M. circinelloides. To clarify this, the gene encoding ME isoform E from Mortierella alpina was homologously expressed. ME overexpression… CONTINUE READING