Role of macromolecular hydration in the binding of the Escherichia coli cyclic AMP receptor to DNA.

@article{Vossen1997RoleOM,
  title={Role of macromolecular hydration in the binding of the Escherichia coli cyclic AMP receptor to DNA.},
  author={K M Vossen and Ralf Wolz and Margaret A. Daugherty and M. G. Fried},
  journal={Biochemistry},
  year={1997},
  volume={36 39},
  pages={11640-7}
}
The osmotic stress technique was used to measure the changes in macromolecular hydration that accompany binding of the Escherichia coli CAP protein to its transcription-regulatory site (C1) in the lactose promoter and that accompany the transfer of CAP from site C1 to nonspecific genomic DNA. Formation of the C1 complex is accompanied by the net release of 79 +/- 11 water molecules. If all water molecules were released from macromolecular surfaces, this result would be consistent with a net… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Similar Papers

Loading similar papers…