Role of keratinocytes in allergic contact dermatitis

  title={Role of keratinocytes in allergic contact dermatitis},
  author={Jonathan N.W.N. Barker},
  journal={Contact Dermatitis},
  • J. Barker
  • Published 1 March 1992
  • Biology, Medicine
  • Contact Dermatitis
Although once thought to play a purely structural role, there is increasing evidence that keratinocytes are actively involved in epidermal immune responses, including allergic contact dermatitis (ACD). In vitro studies demonstrate that both urushiol and nickel sulphate induce cytokine production in cultured keratinocytes including molecules responsible for endothelial cell activation and lymphocyte chemotaxis and adhesion, In vivo, these same molecules are expressed in experimentally induced… 
The immunology of nickel-induced allergic contact dermatitis.
  • W. Sosroseno
  • Biology, Medicine
    Asian Pacific journal of allergy and immunology
  • 1995
Inhibition of NACD can also be generated by feeding with nickel, suggesting that the induction of oral tolerance to nickel may be beneficial for an alternative immunotherapy of nickel allergy, and this testable model provides a direction for further investigation.
Investigation of TNF-alpha release as a measure of skin irritancy.
Dendritic cells and cutaneous immune responses to chemical allergens.
Epidermal cytokines in contact hypersensitivity: Immunological roles and practical applications.
  • I. Kimber
  • Biology, Medicine
    Toxicology in vitro : an international journal published in association with BIBRA
  • 1993
Effect of nickel on the activation state of normal human keratinocytes through interleukin 1 and intercellular adhesion molecule 1 expression
The existence of direct interactions between Ni2+ and keratinocytes, which generate immunological signals of major importance in the pathophysiology of allergic contact dermatitis, are confirmed.
Modification and expulsion of keratins by human epidermal keratinocytes upon hapten exposure in vitro.
A mechanism is proposed where neoepitopes, otherwise hidden from the immune system, are released after hapten exposure via keratinocyte blebbing and the observed expulsion of modified keratins by keratinocytes in vitro might play a role duringHapten sensitization in vivo and should be subject to further investigations.
Cytokine mRNA expression in human epidermis after patch treatment with rhus and sodium lauryl sulfate.
  • C. Ryan, G. Gerberick
  • Medicine, Biology
    American journal of contact dermatitis : official journal of the American Contact Dermatitis Society
  • 1999


Immune Regulation in Allergic and Irritant Skin Reactions
Keratinocytes, which comprise approximately 90% of the total epidermal cell population, are not passive bystanders in the immune response and can transmit both positive and negative signals, thus, they may function as a modulator of the immune system, up or downregulating T-cell responses.
Epidermis and Lymphocyte Interactions During an Allergic Patch Test Reaction. Increased Activity of ETAF/IL-1, epidermal derived lymphocyte reactivity in persons with type IV allergy
All patients with a positive patch test had an increased mixed skin lymphocyte reactivity compared with epidermis coming from a negative reaction, and the ETAF/IL-1 activity in patch test areas was significantly correlated with the clinical response.
Epidermis and lymphocyte interactions during an allergic patch test reaction. Increased activity of ETAF/IL-1, epidermal derived lymphocyte chemotactic factor and mixed skin lymphocyte reactivity in persons with type IV allergy.
The ETAF/IL-1 activity in patch test areas was significantly correlated with the clinical response, and ELCF is not present in epidermis from noneczematous persons.
Keratinocyte expression of MHC class II antigens in allergic sensitization and challenge reactions and in irritant contact dermatitis.
A battery of anti-class II monoclonal antibodies was used in an immunohistochemical study of the sequential changes in the allergic challenge reactions to dinitrochlorobenzene and nickel, the irritant response to anthralin, and the induction of sensitization to DNCB.
Modulation of keratinocyte-derived interleukin-8 which is chemotactic for neutrophils and T lymphocytes.
It is demonstrated that activated human keratinocytes are capable of producing biologically active IL-8, and evidence that keratinocyte-derived products can play a key role in mediating the influx of T cells and neutrophils into the epidermis is provided.
Ia antigen expression on human epidermal Langerhans cells
It is shown that epidermal Langerhans cells react positively by immunofluorescence with antisera raised in rabbits against human B lymphoblastoid cell line membrane glycoproteins, which indicates the presence of Ia-like antigens on these dendritic cells.
Modulation of leucocyte adhesion molecules, a T‐cell chemotaxin (IL‐8) and a regulatory cytokine (TNF‐α) in allergic contact dermatitis (rhus dermatitis)
The results suggest that on exposure to poison ivy/oak, keratinocytes rapidly produce TNF‐α which leads to an early autoinduction of ICAM‐1, and later IL‐8, and there is also a paracrine‐mediated induction and augmentation of underlying endothelial cell ELAM‐2, VCAM‐ 1 and IC AM‐1.
Nickel‐keratinocyte interaction: a possible role in sensitization
The results suggest that the percutaneous penetration of small amounts of Ni2+ can result in damage to keratinocytes and can initiate sensitization.