Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerariaefolium Vis. cell cultures

@article{Hitmi2000RoleOI,
  title={Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerariaefolium Vis. cell cultures},
  author={A. Hitmi and A. Coudret and C. Barthomeuf and H. Sallanon},
  journal={Journal of Plant Physiology},
  year={2000},
  volume={157},
  pages={47-53}
}
  • A. Hitmi, A. Coudret, +1 author H. Sallanon
  • Published 2000
  • Chemistry
  • Journal of Plant Physiology
  • Summary An efficient procedure using sucrose as cryoprotectant to confer freezing tolerance on high-pyrethrinproducing cell lines of Chrysanthemum cinerariaefolium was developed. The efficiency of sucrose depended on both its concentration in the pregrowth medium and on preculture duration. A low sucrose concentration (0.25 mol/L) induced insufficient dehydration of cells, and so failed to prevent damage during freezing and prevented regrowth. The high cell dehydration obtained by incubating… CONTINUE READING
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