• DOI:10.1016/S0176-1617(00)80134-0
Corpus ID: 84054776

Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerariaefolium Vis. cell cultures

@article{Hitmi2000RoleOI,
  title={Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerariaefolium Vis. cell cultures},
  author={A. Hitmi and A. Coudret and C. Barthomeuf and H. Sallanon},
  journal={Journal of Plant Physiology},
  year={2000},
  volume={157},
  pages={47-53}
}

A. Hitmi, A. Coudret, +1 author H. Sallanon

Published 2000

Chemistry

Journal of Plant Physiology

Summary An efficient procedure using sucrose as cryoprotectant to confer freezing tolerance on high-pyrethrinproducing cell lines of Chrysanthemum cinerariaefolium was developed. The efficiency of sucrose depended on both its concentration in the pregrowth medium and on preculture duration. A low sucrose concentration (0.25 mol/L) induced insufficient dehydration of cells, and so failed to prevent damage during freezing and prevented regrowth. The high cell dehydration obtained by incubating… CONTINUE READING

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