Role of intracellular‐free calcium in the cornified envelope formation of keratinocytes: Differences in the mode of action of extracellular calcium and 1,25 dihydroxyvitamin D3
@article{Pillai1991RoleOI, title={Role of intracellular‐free calcium in the cornified envelope formation of keratinocytes: Differences in the mode of action of extracellular calcium and 1,25 dihydroxyvitamin D3}, author={Sreekumar Pillai and Daniel D. Bikle}, journal={Journal of Cellular Physiology}, year={1991}, volume={146} }
Extracellular calcium (Cao) and the steroid hormone 1,25(OH)2D, induce the differentiation of human epidermal cells in culture. Recent studies suggest that increases in intracellular free calcium (Cai) levels may be an initial signal that triggers keratinocyte differentiation. In the present study, we evaluated cornified envelope formation, the terminal event during keratinocyte differentiation, and correlated it with changes in the Cai levels during differentiation of keratinocytes in culture…
144 Citations
1,25 dihydroxyvitamin D3 enhances the calcium response of keratinocytes
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- 1999
It is concluded that 1,25(OH)2D3 maintains the CaR mRNA levels in cells grown in 0.03 mM Ca, thus maintaining their responsiveness to Cao and so ensuring their ability to differentiate in response to the calcium signal.
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The effect of extracellular calcium in raising [Ca2+]i within minutes was more rapid than 1,25(OH)2D3, but in combination the two were not additive.
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Changes in calcium responsiveness and handling during keratinocyte differentiation. Potential role of the calcium receptor.
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The keratinocyte contains a CaR that regulates both the initial release of Cai from intracellular stores and the subsequent increase in calcium flux through nonspecific calcium channels, which may facilitate the maintenance of the high Cai required for differentiation.
Localization and quantitation of calcium pools and calcium binding sites in cultured human keratinocytes
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- 1993
Differences in the distribution of free ionic calcium and organelle‐bound calcium are revealed and the localization pattern of calcium in cultured keratinocytes may reflect the hyperproliferative state of these cells, as in psoriatic epidermis, and the absence of a normal permeability barrier in these submerged cultures.
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- 2003
These studies suggest that at least part of the sequential activation of genes required during keratinocyte differentiation is regulated by the change (availability) of these different coactivator complexes.
Endogenous prostaglandin E2 modulates calcium-induced differentiation in human skin keratinocytes.
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Calcium- and vitamin D-regulated keratinocyte differentiation
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Growth and differentiation stimuli induce different and distinct increases in intracellular free calcium in human keratinocytes
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In normal human keratinocytes an increase in [Ca2+]i appears to be an early event in differentiation, whether induced by calcium or TPA, but not during growth inhibition without differentiation.
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