Role of interferon alpha/beta receptor chain 1 in the structure and transmembrane signaling of the interferon alpha/beta receptor complex.

@article{Constantinescu1994RoleOI,
  title={Role of interferon alpha/beta receptor chain 1 in the structure and transmembrane signaling of the interferon alpha/beta receptor complex.},
  author={Stefan N Constantinescu and E. M. Croze and C Wang and A. S. N. Murti and L Basu and Jerald Eric Mullersman and Lawrence M Pfeffer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 20},
  pages={9602-6}
}
A previously cloned cDNA encodes one subunit of the human interferon alpha/beta receptor (IFN alpha R), denoted IFN alpha R1. To study the expression and signaling of IFN alpha R1, we used monoclonal antibodies (mAbs) generated against the baculovirus-expressed ectodomain of IFN alpha R1. Immunoprecipitation and immunoblotting of lysates from a variety of human cell lines showed that IFN alpha R1 has an apparent molecular mass of 135 kDa. Binding analysis with 125I-labeled mAb demonstrated high… CONTINUE READING