Role of glycerol‐3‐phosphate dehydrogenase 2 in mouse sperm capacitation

@article{Kota2010RoleOG,
  title={Role of glycerol‐3‐phosphate dehydrogenase 2 in mouse sperm capacitation},
  author={Venkatesh Kota and Priyank Rai and Joachim M. Weitzel and Ralf Middendorff and Satish S. Bhande and Sisinthy Shivaji},
  journal={Molecular Reproduction and Development},
  year={2010},
  volume={77}
}
A tyrosine phosphoproteome study of hamster spermatozoa indicated that glycerol‐3‐phosphate dehydrogenase 2 (GPD2), is one of the proteins that enables tyrosine phosphorylation during sperm capacitation. Further, enzymatic activity of GPD2 correlated positively with sperm capacitation [Kota et al., 2009 ; Proteomics 9:1809–1826]. Therefore, understanding the function of GPD2 would help to unravel the molecular mechanism of sperm capacitation. In this study, involving the use of spermatozoa from… 
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References

SHOWING 1-10 OF 56 REFERENCES
Tyrosine phosphoproteome of hamster spermatozoa: Role of glycerol‐3‐phosphate dehydrogenase 2 in sperm capacitation
TLDR
Enzymatic activity of GPD2 during capacitation correlates positively with hyperactivation and acrosome reaction thus demonstrating that GPD1 may be required for sperm capacitation and attempts have been made to ascertain the role of one of these eleven proteins namely glycerol‐3‐phosphate dehydrogenase 2 (GPD2) in hamster sperm capacitate.
Activity of Pyruvate Dehydrogenase A (PDHA) in Hamster Spermatozoa Correlates Positively with Hyperactivation and Is Associated with Sperm Capacitation1
TLDR
Given the localization of PDHA and the evidence that its activity correlates positively with hyperactivation and that its PDHA2 subunit exhibits capacitation-associated protein tyrosine phosphorylation, it appears thatPDHA2 is associated with the process of capacitation.
Hamster Sperm Capacitation: Role of Pyruvate Dehydrogenase A and Dihydrolipoamide Dehydrogenase1
TLDR
It is established that PDHA is required for hamster sperm hyperactivation and acrosome reaction, and DLD is required in the presence of BP and MICA, and ROS, cAMP, and calcium are involved downstream to PDHA.
Regulation of protein-tyrosine phosphorylation and human sperm capacitation by reactive oxygen derivatives.
Novel Tyrosine-Phosphorylated Post-Pyruvate Metabolic Enzyme, Dihydrolipoamide Dehydrogenase, Involved in Capacitation of Hamster Spermatozoa1
TLDR
This report identifies a candidate protein, dihydrolipoamide dehydrogenase, which is a post-pyruvate metabolic enzyme, exhibiting tyrosine phosphorylation during hamster spermatozoal capacitation, and delineates the temporal involvement of glucose and pyruVate-lactate, showing that the former is required in the earlier stages and the latter for the later stages of hamster infertility capacitation.
Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation.
TLDR
It is hypothesized that tyrosine phosphorylation of these proteins can play a role in the regulation of glycolysis during capacitation, but neither the Km nor the Vmax of aldolase changed as a function of capacitation when its enzymatic activity was assayed in vitro, suggesting other levels of regulation for aldlase function.
Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.
  • K. Miki, W. Qu, +6 authors D. A. O’Brien
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 2004
TLDR
The critical role of glycolysis in sperm and its dependence on this sperm-specific enzyme suggest that GAPDS is a potential contraceptive target, and that mutations or environmental agents that disrupt its activity could lead to male infertility.
Phosphoproteome Analysis of Capacitated Human Sperm
TLDR
The phosphopeptide enrichment and quantification methodology coupled to MS/MS, described here for the first time, can be employed to map and compare phosphorylation sites involved in multiple cellular processes.
Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function.
TLDR
The redox status of human spermatozoa was found to have a profound influence on the fertilizing potential of these cells in association with qualitative and quantitative changes in the patterns of tyrosine phosphorylation, and the fact that the biological responses of human infertility cells to biological agonists could also be inhibited by catalase indicated the general relevance of these findings.
Identification of the major tyrosine phosphorylated protein of capacitated hamster spermatozoa as a homologue of mammalian sperm a kinase anchoring protein
TLDR
Evidence is provided that two major tyrosine phosphorylated proteins of molecular weight 97 and 83 kDa are the hamster homologues of mouse pro‐AKAP82 and AKAP82, and have been designated as hamster pro-AKAP83 and AKap83 respectively.
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