Role of glutathione in cell survival after hyperthermic treatment of Chinese hamster ovary cells.

@article{Freeman1985RoleOG,
  title={Role of glutathione in cell survival after hyperthermic treatment of Chinese hamster ovary cells.},
  author={Michael L Freeman and Arnold W. Malcolm and Michael J. Meredith},
  journal={Cancer research},
  year={1985},
  volume={45 12 Pt 1},
  pages={6308-13}
}
Chinese hamster ovary cells cultured in vitro were exposed to hyperthermic treatment at 43 degrees C or 45 degrees C. Depletion of 99.5% of the intracellular glutathione (GSH) by addition of 0.1 mM diethylmaleate (DEM) increased thermal sensitivity. This effect, however, was dependent upon oxygen tension. Decreasing the oxygen tension reduced the effect. Thermal cytotoxicity was further augmented by continued depletion of GSH after hyperthermic treatment. Once cells became thermally tolerant… CONTINUE READING