Role of gel test and flow cytometry in diagnosis of Coombs' negative autoimmune haemolytic anaemia.

@article{Fayek2012RoleOG,
  title={Role of gel test and flow cytometry in diagnosis of Coombs' negative autoimmune haemolytic anaemia.},
  author={Manal Hashem Fayek and Amr A. Saad and Doaa G Eissa and Lamis Mohamed Tawfik and Geeta Kamal},
  journal={International journal of laboratory hematology},
  year={2012},
  volume={34 3},
  pages={311-9}
}
INTRODUCTION This study evaluated the use of both gel test (GT) and flow cytometry (FC) techniques in the detection of red blood cell-bound immunoglobulin G (IgG) and, hence, the diagnosis of autoimmune haemolytic anaemia (AIHA) in cases that were negative by the conventional tube technique (CTT) direct antiglobulin test (DAT). METHODS The study enrolled 50 clinically diagnosed AIHA patients with CTT Coombs' negative test and 55 control subjects. GT and FC were performed on both groups… CONTINUE READING
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