Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro.

@article{Borowczyk2007RoleOG,
  title={Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro.},
  author={Ewa Borowczyk and Mary Lynn Johnson and Jerzy J. Bilski and Magda A Bilska and Dale A. Redmer and Lawrence P Reynolds and Anna T. Grazul-Bilska},
  journal={Reproduction},
  year={2007},
  volume={133 3},
  pages={641-51}
}
To evaluate the role of gap junctions in the regulation of progesterone secretion, two experiments were conducted. In Experiment 1, luteal cells obtained on days 5, 10, and 15 were cultured overnight at densities of 50 x 10(3), 100 x 10(3), 300 x 10(3), and 600 x 10(3) cells/dish in medium containing: (1) no treatment (control), (2) LH, or (3) dbcAMP. In Experiment 2, luteal cells from days 5 and 10 of the estrous cycle were transfected with siRNA, which targeted the connexin (Cx) 43 gene. In… CONTINUE READING
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