Role of epidermal growth factor in bovine oocyte maturation and preimplantation embryo development in vitro.

@article{Lonergan1996RoleOE,
  title={Role of epidermal growth factor in bovine oocyte maturation and preimplantation embryo development in vitro.},
  author={Pat Lonergan and Catherine Carolan and Anne van Langendonckt and Isabelle Donnay and Hadjer Khatir and Pascal Mermillod},
  journal={Biology of reproduction},
  year={1996},
  volume={54 6},
  pages={
          1420-9
        }
}
Epidermal growth factor (EGF) has been shown to have a positive effect during in vitro maturation (IVM) and has been reported in follicular fluid at levels capable of stimulating meiosis in a variety of species. The aim of the present work was to study the effect on subsequent development of EGF present in defined medium during bovine 1) oocyte maturation or 2) embryo culture. The presence of EGF during IVM, irrespective of concentration (1, 10, 100 ng/mg), stimulated cumulus expansion and… 

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Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro.

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References

SHOWING 1-10 OF 71 REFERENCES

Epidermal growth factor enhances oocyte maturation in pigs

It is concluded that EGF alone can stimulate nuclear maturation in pig oocytes, and EGF can interact with gonadotropins to enhance cytoplasmic maturation.

Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in vitro by the addition of epidermal growth factor and insulin-like growth factor I.

EGF, either alone or together with IGF-I, stimulates cumulus expansion; and both growth factors, acting alone or Together, enhance nuclear maturation in oocytes surrounded by compact cumulus cells.

Bovine blastocyst development after in vitro maturation in a defined medium with epidermal growth factor and low concentrations of gonadotropins.

A possible physiological role for EGF in regulating bovine oocyte maturation is suggested, as combination of EGF with low concentrations of gonadotropins during IVM enabled subsequent blastocyst development in proportions comparable to those afforded by high concentrations of FSH or LH.

Development of bovine embryos in vitro following oocyte maturation under defined conditions.

Both tissue culture medium 199 (M199) and synthetic oviduct fluid (SOF) are capable of supporting the IVM of bovine oocytes at high rates in the absence of macromolecular supplements, as evidenced by subsequent development to the blastocyst stage.
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