Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes.

@article{RmyKristensen2001RoleOE,
  title={Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes.},
  author={A. R{\'e}my-Kristensen and Jean Pierre Clamme and Christine Vuilleumier and J. G. Kuhry and Yves M{\'e}ly},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1514 1},
  pages={21-32}
}
Polyethylenimine (PEI) is one of the most efficient nonviral vectors for gene therapy. The aim of this study was to investigate the role of endocytosis in the transfection of synchronized L929 fibroblasts by PEI/DNA complexes. This was performed by confocal microscopy and flow cytometry, using the endocytosis marker FM4-64 and PEI/DNA complexes labeled either with the DNA intercalator YOYO-1, or with fluorescein covalently linked to PEI. Endocytosis appeared as the major if not the sole mode of… CONTINUE READING