Role of ecNOS-derived NO in mediating TNF-induced endothelial barrier dysfunction.

@article{Bove2001RoleOE,
  title={Role of ecNOS-derived NO in mediating TNF-induced endothelial barrier dysfunction.},
  author={Kathleen Bove and Paul H Neumann and Nancy Gertzberg and A. Johnson},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={2001},
  volume={280 5},
  pages={L914-22}
}
We tested the hypothesis that endothelial cell nitric oxide synthase (ecNOS) mediates the tumor necrosis factor (TNF)-alpha-induced increase in nitric oxide (NO) and albumin permeability in pulmonary microvessel endothelial monolayers (PEM). PEM lysates were analyzed for ecNOS mRNA (RT-PCR), ecNOS protein (Western immunoblot), NO levels (NO, the oxidation product of NO), and barrier function (albumin clearance rate). PEM were incubated with TNF (50 ng/ml) for 0.5, 2, 4, and 24 h. TNF induced a… CONTINUE READING
24 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 24 extracted citations

Similar Papers

Loading similar papers…