Role of disulfides in biological activity and conformational stability of pig kidney diamine oxidase: evidence for two disulfide states.


Six disulfides are found to be present in pig kidney diamine oxidase and all of these are available to reducing agents under nondenaturating conditions. Disulfide reduction with dithiothreitol followed by carbamidomethylation indicated two states of disulfides, each containing three groups, distinguishable by pH dependence. The first group of three… (More)