Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification.

Abstract

The role of the three cysteine residues at positions 13, 63 and 133 in Escherichia coli RNAase H, an enzyme that is sensitive to N-ethylmaleimide [Berkower, Leis & Hurwitz (1973) J. Biol. Chem. 248, 5914-5921], was examined by using both site-directed mutagenesis and chemical modification. Novel aspects that were found are as follows. First, none of the… (More)

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@article{Kanaya1990RoleOC, title={Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification.}, author={Shigenori Kanaya and S-i Kimura and Chie Katsuda and Michio Ikehara}, journal={The Biochemical journal}, year={1990}, volume={271 1}, pages={59-66} }