Role of cholesterol as a structural and functional effector of the nicotinic acetylcholine receptor.
@article{FernndezBallester1994RoleOC, title={Role of cholesterol as a structural and functional effector of the nicotinic acetylcholine receptor.}, author={Gregorio Fern{\'a}ndez-Ballester and Jose Castresana and A. M. Fern{\'a}ndez and Jos{\'e} L. R. Arrondo and Jos{\'e} Antonio Ferragut and Jos{\'e} Manuel Gonz{\'a}lez-Ros}, journal={Biochemical Society transactions}, year={1994}, volume={22 3}, pages={ 776-80 } }
776 Role of cholesterol as a structural and functional effector of the nicotinic acetylcholine receptor Gregorio Fernandez-Ballester*, Jose Castresanat, Asia M. Fernandez*, Jose-Luis R. Arrondot, Jose A. Ferragut* and Jose M. Gonzalez-Ros*$ *Department of Neurochemistry and Institute of Neurosciences, University of Alicante, 03080 Alicante, Spain and tDepartrnent of Biochemistry, Faculty of Sciences, University of the Basque Country, 48080 Bilbao, Spain
16 Citations
Structural and functional changes induced in the nicotinic acetylcholine receptor by membrane phospholipids
- Biology, ChemistryJournal of Molecular Neuroscience
- 2007
Re reconstituted purified AChRs from Torpedo into complex multicomponent lipid vesicles in which the phospholipid composition has been systematically altered to postulation of a possible role of certain lipids as peculiar allosteric ligands of the protein.
Modulation of nicotinic acetylcholine receptor function through the outer and middle rings of transmembrane domains.
- BiologyCurrent opinion in drug discovery & development
- 2003
A major challenge for the future is to elucidate the mechanism of propagation of information from the lipid-exposed TM rings to the rest of the receptor molecule.
Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane
- Biology, ChemistryInternational journal of Alzheimer's disease
- 2011
Circular Dichroism (CD) spectroscopy shows that after 24 and 48 hours of incubation with AβP1-40, choline stabilizes the random coil conformation of the peptide, making it less prone to fibrillate, and these actions seem to be specific in that ACh is ineffective either in solution or on A βP 1-40 channel incorporated into PLMs.
Uncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies
- Biology, ChemistryScientific reports
- 2016
This study compares the lipid composition, including individual phospholipid molecular species of solubilized nAChR detergent complexes with those of the bulk lipids from their source, Torpedo californica electric tissue, and found that upon incorporation into oocytes, FC12 produce significant functionality, whereas LFC14 and LFC16 nA ChR-DCs displayed an increased functionality as compared to the crude Tc membrane.
Unique Effects of Different Fatty Acid Species on the Physical Properties of the Torpedo Acetylcholine Receptor Membrane*
- Biology, ChemistryThe Journal of Biological Chemistry
- 2002
It is suggested that it is the direct action of FFA at the lipid-protein interface, displacing essential lipids from their sites rather than changes in bulk properties such as membrane fluidity that accounts for the effect of F FA on the acetylcholine receptor membrane.
Lipids modulate the conformational dynamics of a secondary multidrug transporter
- BiologyNature Structural &Molecular Biology
- 2016
The model emerging from the data suggests a direct interaction between lipid headgroups and a conserved motif of charged residues that control the conformational equilibrium through an interplay of electrostatic interactions within the protein.
Role of Cholesterol in Formation and Function of a Signaling Complex Involving αvβ3, Integrin-Associated Protein (Cd47), and Heterotrimeric G Proteins
- Biology, ChemistryThe Journal of cell biology
- 1999
Data demonstrate that cholesterol is an essential component of the αvβ3/IAP/G protein signaling complex, presumably acting through an effect on IAP conformation.
Role of Cholesterol in Formation and Function of a Signaling Complex Involving (cid:97) v (cid:98) 3, Integrin-associated Protein (CD47), and Heterotrimeric G Proteins
- Biology, Chemistry
- 1999
It is demonstrated that cholesterol is an essential component of the ( cid:97) v (cid:98) 3/ IAP/G protein signaling complex, presumably acting through an effect on IAP conformation.
Lateral Diffusion, Function, and Expression of the Slow Channel Congenital Myasthenia Syndrome αC418W Nicotinic Receptor Mutation with Changes in Lipid Raft Components*
- Biology, MedicineThe Journal of Biological Chemistry
- 2015
The findings support the hypothesis that a cholesterol-sensitive nAChR might reside in specialized membrane microdomains that upon cholesterol depletion become disrupted and release the cholesterol- sensitive nA ChRs to the pool of activable receptors.
Alzheimer’s Disease as a Membrane Disorder: Spatial Cross-Talk Among Beta-Amyloid Peptides, Nicotinic Acetylcholine Receptors and Lipid Rafts
- Biology, ChemistryFront. Cell. Neurosci.
- 2019
The implications of lipid-protein interactions at the cell membrane level in AD are discussed and Aβ processing, Aβ partitioning, and acetylcholine receptor location and function can be manipulated by changes in membrane lipid biophysics.
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