• Biology
  • Published 2006

Role of caspase-3 played in As_4S_4 induced apoptosis of Hela cell line

@inproceedings{Demin2006RoleOC,
  title={Role of caspase-3 played in As_4S_4 induced apoptosis of Hela cell line},
  author={Pu De-min},
  year={2006}
}
Objective To investigate the possible role of caspase-3 and bcl-2 played in As_4S_4 induced apoptosis of cervical tumor Hela cell line.Method Hela cells were cultured with various concentrations(7.5,15,30 and 60 mg/L)of As_4S_4 for 12,24,36,48 and 60 h respectively,then measuring the situation of cell growth apoptosis with MTT and flow cytometry analysis.The expressions of(bcl-2) and caspase-3 protein were detected by Western blot,caspase-3 activity was evaluated with flow cytometry.Result The… CONTINUE READING