Role of calcium influx in cytotoxic T lymphocyte lytic granule exocytosis during target cell killing.

@article{Lyubchenko2001RoleOC,
  title={Role of calcium influx in cytotoxic T lymphocyte lytic granule exocytosis during target cell killing.},
  author={Taras A Lyubchenko and Georjeana A Wurth and Adam Zweifach},
  journal={Immunity},
  year={2001},
  volume={15 5},
  pages={847-59}
}
One mechanism cytotoxic T lymphocytes use to kill targets is exocytosis of cytotoxic agents from lytic granules, a process that requires Ca(2+) influx. We investigated the role of Ca(2+) influx in granule exocytosis using TALL-104 human leukemic cytotoxic T cells triggered via a bispecific antibody containing an anti-CD3 F(ab') to kill Raji B lymphoma cells. Using a novel fluorescence method, we detected target-directed release of approximately 15% of lytic granules during killing. Consistent… CONTINUE READING